New Delhi Metallo-β-lactamase in Enterobacteriaceae species isolated from hospitalized patients, Managua Nicaragua

Abstract

Objective: to determine the frequency of New Delhi-type metallo-β-lactamase (NDM) in isolates of enterobacteria from patients hospitalized with different infectious processes. Method: a cross-sectional descriptive study was carried out between August 2015 and October 2016 at Alemán Nicaragüense Hospital. A total of 249 strains were studied in active surveillance of carbapenems resistance. The identification and resistance profile was carried out in Vitek2. Suspected resistance to carbapenems was considered when the MIC of Imipenem and Meropenem was 2-4 μg/mL and for Ertapenem of 2 μg/mL, determined by Kirby Bauer, the triple disc synergy test (carbapenems and EDTA 10μg). A polymerase chain reaction test was made to determinate New Delhi metallo-β lactamase. Results: a total of 249 strains were analyzed, among which 45 strains resistant to carbapenems were identified, corresponding to 18%. Of these strains, 43 were positive for the synergy test with EDTA; 21 carried the New Delhi gene. Of the New Delhi metallo-β lactamase. 66% were found in isolates of Klebsiella pneumoniae, followed by Escherichia vulneris in 6 isolates, Escherichia coli in 2, Providencia rettgeri in 2, Pantoea agglomerans in 2 and Kluyvera cryocrescens by 2. Conclusions: the results of the present study are a clear warning about the circulation of New Delhi-type metallo-β-lactamase strains that codify for the resistance to carbapenems in the hospital analyzed.